De-Ionised Water for Analytical Use in Laboratories
What is De-Ionised Water and Its Importance in Analytical Chemistry?
De-ionised water (DI water) is water that has had its mineral ions removed, including sodium, calcium, iron, and copper. Ion exchange replaces unwanted ions with hydrogen and hydroxyl ions to create pure H2O. DI water is essential in analytical chemistry and laboratory applications due to its high purity.
The significance of DI water in analytical chemistry is immense. Using impure water in experiments can lead to contamination, resulting in inaccurate results. Even trace amounts of ions can alter chemical reactions and distort measurements. DI water ensures that lab results remain valid, reliable, and free from external influences that could compromise the accuracy of experiments.
Key applications of DI water in laboratories include:
- Reagent preparation: Ensures the purity of chemical solutions.
- Sample dilution: Prevents contaminants that could affect concentrations.
- Rinsing equipment and glassware: Avoids residue that may interfere with future procedures.
As laboratory methods become more sensitive, the demand for high-purity water rises. De-ionised water plays a crucial role in ensuring experimental accuracy and reliability in analytical chemistry.
Best Practices for Handling and Storing DI Water in Laboratories
Maintaining the purity of de-ionised water is essential for its effectiveness in analytical procedures. Following best practices in handling and storage is critical to preserving its quality.
Key Practices:
- Use proper containers: Store de-ionised water in HDPE or fluorinated containers, which prevent contaminants from leaching into the water.
- Minimize air exposure: Store water in airtight containers to prevent re-ionization.
- Use dedicated equipment: Transfer water using clean, dedicated tools like pipettes, to avoid contamination.
- Test regularly: Establish a protocol to monitor the water’s conductivity and total dissolved solids (TDS). Document results for quality control and compliance.
By following these guidelines, laboratories can ensure reliable, reproducible results. Proper handling of DI water supports the accuracy and integrity of scientific research, making it indispensable for analytical work.
